出版時間:2008-10 出版社:西南師范大學(xué)出版社 作者:夏慶友,向仲懷 編 頁數(shù):651
內(nèi)容概要
這本研究論文集總結(jié)8年來中國家蠶基因組計劃的主要研究結(jié)果,為已經(jīng)啟動并有著美好前景的家蠶功能基因組計劃提供借鑒和參考。全書分為四個部分,收錄了這個期間正式發(fā)表的論文共計68篇,分別反映中國家蠶基因組計劃之背景、意義和策略(4篇);測序、生物信息學(xué)和基因組分析(15篇);關(guān)鍵平臺技術(shù)(17篇)和功能基因組及其應(yīng)用(31篇)等。
書籍目錄
家蠶基因組研究對蠶業(yè)學(xué)科和產(chǎn)業(yè)發(fā)展的影響(代序)前言第一部分 背景、意義和策略21世紀(jì)的絲綢之路國際鱗翅目昆蟲基因組筑波會議與中國家蠶基因組為絲綢之路重添異彩關(guān)于盡快啟動“中國家蠶基因組計劃”的建議中國家蠶基因組與21世紀(jì)絲綢之路第二部分 測序、生物信息學(xué)和基因組分析A draft sequence for the genome of the domesticated silkworm (Bombyw mori)家蠶(Bombyx mori ) 全基因組框架圖SilkDB:a knowledgebase for silkworm biology and genomicsEST-based profiling and comparison of gene expression in the silkworm fat body during metamorphosisMining single nucleotide polymorphisms from EST data of silkworm, Bombyx mori , inbred strain DazaoDetection and analysis of alternative splicing in the silkworm by aligning expressed sequence tags with the genomic sequenceAnalysis on frequency and density of microsatellites in coding sequences of several eukaryotic genomesSurvey of long terminal repeat retrotransposons of domesticated silkworm(Bombyx mori )家蠶cDNA文庫構(gòu)建及大規(guī)模EST測序家蠶母性基因的表達(dá)序列標(biāo)簽分析家蠶chi、gluE和fruA基因與微生物相應(yīng)基因的同源性及基因水平轉(zhuǎn)移初探家蠶細(xì)胞色素P450的基因組學(xué)分析家蠶脂肪體組織基因表達(dá)譜的研究工.家蠶5齡中期幼蟲脂肪體組織基因表達(dá)分析家蠶線粒體基因組1.8kb片段的克隆及序列分析家蠶線粒體基因組全序列測定與分析第三部分 關(guān)鍵平臺技術(shù)Microarray-based gene expression profiles in multiple tissues of the domesticated silkworm, Bombyx moriIdentification and characterization of piggyBac-like elements in the genome of domesticated silkworm, Bombyx moriStudies on middle and posterior silk glands of silkworm (Bombyx mori) using two-dimensional electrophoresis and mass spectrometryEstablishment and characterization of two embryonic cell lines of Bombyx mori利用精子介導(dǎo)法向蠶卵導(dǎo)人外源基因的研究家蠶轉(zhuǎn)基因載體pBacA3EG的構(gòu)建及其表達(dá)GAL4/UAS系統(tǒng)在轉(zhuǎn)基因技術(shù)中的應(yīng)用研究進(jìn)展紅色熒光標(biāo)記載體pBacA4DsRed的構(gòu)建及其在蠶卵中的瞬時表達(dá)家蠶轉(zhuǎn)基因載體pBacA3EG在家蠶培養(yǎng)細(xì)胞中的表達(dá)家蠶絲腺蛋白質(zhì)組學(xué)研究方法的建立家蠶中腸組織蛋白質(zhì)組學(xué)研究家蠶胚胎發(fā)育時期的RNA干涉研究一種家蠶卵巢原代細(xì)胞制作方法探索家蠶胚胎細(xì)胞系的DNA指紋圖譜分析家蠶胚胎細(xì)胞系BmE—SWU2的建立及其生物學(xué)特性研究家蠶脂肪體組織原代培養(yǎng)及其細(xì)胞分化的研究CELI酶的粗純化及活性分析第四部分 功能基因組及其應(yīng)用Structures, regulatory regions, and inductive expression patterns of antimicrobial peptide genes in the silkworm Bombyx moriProteolytic activation of pro-spfitzle is required for the induced transcription of antimicrobial peptide genes in lepidopteran insectsIdentification and analysis of Toll-related genes in the domesticated silkworm, Bombyx moriCharacterization and expression patterns of let-7 microRNA in the silkworm (Bombyx mori )Analysis of the structure and expression of the 30K protein genes in silkworm, Bombyx moriPurification, characterization and cloning of a chymotrypsin inhibitor (CI-9) from the hemolymph of the silkworm, Bombyx moriIdentification of three annexin IX isoforms generated by alternative splicing of the carboxyl-terminal exon in silkworm, Bombyx moriAnalysis of a Novel Transcription Mode of Fhx/P25 Gene in Bombyx moriIdentification and expression pattern of Bmlark, a homolog of the Drosophila gene lark in Bombyx moriMolecular cloning and characterization of Bombyx mori sterol carrier protein x/sterol carrier protein 2 (SCPx/SCP2) geneIn silico identification of silkworm selenoproteomicsIdentification and expression pattern of the chemosensory protein gene family in the silkworm, Bombyx moriSequence structure and expression pattern of a novel anionic defensin-like gene from silkworm (Bombyx mori)昆蟲性別決定的分子機(jī)制研究進(jìn)展家蠶sans fille基因的分子克隆及序列分析家蠶血液胰凝乳蛋白酶抑制劑的多態(tài)性分布一個新的家蠶Kunitz類型CI基因的克隆和序列分析家蠶卵黃原蛋白及其受體基因家蠶組織蛋白酶D基因的克隆、序列分析及其表達(dá)譜研究中國野桑蠶性信息素合成激活肽(PBAN)基因的克隆及序列分析家蠶信息素結(jié)合蛋白BmPBP2和BmPBP3基因的初步鑒定及表達(dá)分析
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